MabPolish^TM DUO

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Introduction

MabPolish DUO is a class of novel resins with inert shell in the outer-layer of the bead (see the diagram below). The shell has size-exclusion effect that blocks molecules based on their molecular weights. Impurities smaller than a mAb can penetrate the shell and then be captured by the mixed-mode ligand inside the bead. It is a very gentle method with little loss of product.

The figure above shows the effectiveness of blocking IgG by the size-exclusion shell.

	Key Features	Application Guide
MabPolishTM DUO 150A MabPolishTM DUO 400A	Shelled anion mixed-mode resin. The shell of 150A can block molecules of 150 KDa. The shell of 400A can block molecules around 400 KDa	Flow-through mode to capture impurities smaller than IgGs at a wide range of pH and conductivity. MabPolish DUO 400 range can be run at faster flow velocity to remove high molecular weight species with low loss of target antibodies.
MabPolishTM DUO 150C MabPolishTM DUO 400C	Shelled cation mixed-mode resin. The shell of 150C can block molecules of 150 KDa. The shell of 400C can block molecules around 400 KDa.

MabPolish DUO can be used in flow-through mode to remove impurities of less than 150 KDa from monoclonal antibodies or other antibody materials. Examples of the possible application scenario could be:

• After an antibody is first purified by affinity chromatography media (e.g. Protein A, Protein G or Protein L etc);
• After an antibody is first purified by ion-exchangers;
• After an antibody is first processed through precipitation;
• Direct removal of impurities from crude antibody materials
• After an antibody is modified (e.g. conjugated etc)

Characteristics of MabPolish DUO

Matrix	Beads of cross-linked polysaccharide composite
Particle size	50 – 200 µm
Functional group	150A and 400A: anion mixed mode ligand 150C and 400C: cation mixed mode ligand
Size exclusion effect of the out-layer	150A and 150C: excluding 150 KDa molecules 400A and 400C: excluding 400 KDa molecules
Operational pressure	Up to 3 bar
pH stability	2-14 (short term) and 3-12 (long term)
Working temperature	+4oC to +30oC
Chemical stability	All commonly used buffers
Avoid	Oxidizing agents, detergents

Ordering information

Ordering information

Product	Quantity	Code no.	Price GBP
MabPolish DUO 150A	25 ml	270301-25ML	£186.90
	100 ml	270301-100ML	POR
	1 litre	270301-1L	POR
Pre-packed column	5 x 1 ml	270301-5x1ML	£179.00
	1 x 10 ml	270301-1x10ML	£245.70
MabPolish DUO 150C	25 ml	270302-25ML	£186.90
	100 ml	270302-100ML	POR
	1 litre	270302-1L	POR
Pre-packed column	5 x 1 ml	270302-5x1ML	£179.00
	1 x 10 ml	270302-1x10ML	£245.70
MabPolish DUO 400A	25 ml	270303-25ML	£186.90
	100 ml	270303-100ML	POR
	1 litre	270303-1L	POR
Pre-packed column	5 x 1 ml	270303-5x1ML	£179.00
	1 x 10 ml	270303-1x10ML	£245.70
MabPolish DUO 400C	25 ml	270304-25ML	£186.90
	100 ml	270304-100ML	POR
	1 litre	270304-1L	POR
Pre-packed column	5 x 1 ml	270304-5x1ML	£179.00
	1 x 10 ml	270304-1x10ML	£245.70
MabPolish Selection Kit	6 x 1 ml (1 ml each of MabPolish Type I, Type II, MabPolish DUO 150A, 150C, 400A, 400C)	270000-6x1ML	£198.45

*Other column size available on request.

How to order